The use of the multidimensional protein identification technology (MudPIT) to analyze plasma proteome of astronauts collected before, during, and after spaceflights

We applied one of the highly sensitive proteomic approaches, i.e. Multidimensional Protein Identification Technology (MudPIT), to determine protein alterations, qualitatively and quantitatively in plasma samples collected before, during, after spaceflights from same astronauts. These were prepared blood drawn acid-citrate dextrose (ACD) tubes. Samples four recruited astronauts available for analysis. There two sampling time-points each stage spaceflight, resulting six astronaut The results would help identify proteins that are candidates functional roles response spaceflight or re-adaptation earth's environment. in-flight duration was 6 months. A total 453 unique non-redundant identified at > 99% confidence. found 19 with significantly altered expression due spaceflights. include lumican, extracellular matrix 1, vitronectin, filaggrin-2, ceruloplasmin, desmoplakin, antithrombin-III, fibronectin, disulfide-isomerase A3, titin, tropomodulin-3, zyxin, bridging integrator 2, apolipoprotein A-II, platelet factor 4 variant, beta-Ala-His dipeptidase (or carcinosine dipeptidase), alpha-2-HS-glycoprotein, pigment epithelium-derived factor, CD5 antigen-like. involved inflammatory responses, cytoskeleton system, metabolism. MudPIT method also allows detection several low-abundance astronauts' have not been reported. Importantly, such may potentially Our provide a significant foundation future studies larger cohort will increase our knowledge space biology enable development countermeasures.